Entrapment of bacteria in fluid inclusions in laboratory-grown halite

Cells of the bacterium Pseudomonas aeruginosa, which were genetically modified to produce green fluorescent protein, were entrapped in fluid inclusions in laboratory-grown halite. The bacteria were used to inoculate NaCl-saturated aqueous solutions, which were allowed to evaporate and precipitate halite. The number, size, and distribution of fluid inclusions were highly variable, but did not appear to be affected by the presence of the bacteria. Many of the inclusions in crystals from inoculated solutions contained cells in populations ranging from two to 20. Microbial attachment to crystal surfaces was neither evident nor necessary for entrapment. Cells occurred exclusively within fluid inclusions and were not present in the crystal matrix. In both the inclusions and the hypersaline solution, the cells fluoresced and twitched, which indicates that the bacteria might have remained viable after entrapment. The fluorescence continued up to 13 months after entrapment, which indicates that little degradation of the bacteria occurred over that time interval. The entrapment, fluorescence, and preservation of cells were independent of the volume of hypersaline solution used or whether the solutions were completely evaporated prior to crystal extraction. The results of this study have a wide range of implications for the long-term survival of microorganisms in fluid inclusions and their detection through petrography. The results also demonstrate the preservation potential for microbes in hypersaline fluid inclusions, which could allow cells to survive harsh conditions of space, the deep geologic past, or burial in sedimentary basins.     

Planetary Radiation Budgets

The energy state of a planet depends fundamentally on its radiation budget. Measurements made from space over past decades have led to significant revisions of ground-based estimates, both of the reflected fraction (the Bond albedo) of solar radiative flux and of the emitted thermal infrared radiation flux, for the Earth as well as for the other planets. After a brief survey of methods and difficulties in accurately determining planetary radiation budgets, we note contradictions in existing tabulations of global parameters, in particular Bond albedo. For the Earth, such contradictions are unjustified, considering that global and annual means as well as the seasonal cycle of Earth Radiation Budget components have now been determined with high accuracy. The Earth's Bond albedo is close to 0.3. Net storage of energy in the Earth-ocean system is close to zero, with a well-established annual cycle of amplitude close to ±12 Wm⁻². Some contradictions remain for the other terrestrial planets. For the giant planets, modern reduced values of the Bond albedo imply reduced but still significant internal energy generation.     

Use of Optical Characteristics to Identify Orbiting Material

Knowledge of the observable properties of orbital debris is necessary to validate debris models for both the low Earth orbit (LEO) and the geosynchronous Earth orbit (GEO). Current methods determine the size and mass of orbital debris based on knowledge or assumption of the material type of the piece. Improvement in the knowledge of material is the goal of the research described herein. The process of using spectral absorption features to determine the material type is explored. A review of the optical measurements of orbital debris as well as current research in the area is discussed. Reflectances of common spacecraft materials are compared. The need for, and advances made possible by obtaining real data are explored. The prospects of the venture are investigated.     

The Ultraviolet Spectrograph on NASA’s Juno Mission

The ultraviolet spectrograph instrument on the Juno mission (Juno-UVS) is a long-slit imaging spectrograph designed to observe and characterize Jupiter’s far-ultraviolet (FUV) auroral emissions. These observations will be coordinated and correlated with those from Juno’s other remote sensing instruments and used to place in situ measurements made by Juno’s particles and fields instruments into a global context, relating the local data with events occurring in more distant regions of Jupiter’s magnetosphere. Juno-UVS is based on a series of imaging FUV spectrographs currently in flight—the two Alice instruments on the Rosetta and New Horizons missions, and the Lyman Alpha Mapping Project on the Lunar Reconnaissance Orbiter mission. However, Juno-UVS has several important modifications, including (1) a scan mirror (for targeting specific auroral features), (2) extensive shielding (for mitigation of electronics and data quality degradation by energetic particles), and (3) a cross delay line microchannel plate detector (for both faster photon counting and improved spatial resolution). This paper describes the science objectives, design, and initial performance of the Juno-UVS.     

How Galactic Cosmic Ray models affect the estimation of radiation exposure in space

The radiation environment in space is a major concern for human spaceflight because of the adverse effects of high levels of radiation on astronauts’ health. Therefore, it is essential to perform radiation risk assessments already during the concept studies of a manned mission. Galactic Cosmic Rays (GCR) have been identified to be one of the primary sources of radiation exposure in space.     

Modeling of Venus, Mars, and Titan

Increased computer capacity has made it possible to model the global plasma and neutral dynamics near Venus, Mars and Saturn??s moon Titan. The plasma interactions at Venus, Mars, and Titan are similar because each possess a substantial atmosphere but lacks a global internally generated magnetic field. In this article three self-consistent plasma models are described: the magnetohydrodynamic (MHD) model, the hybrid model and the fully kinetic plasma model. Chamberlain and Monte Carlo models of the Martian exosphere are also described. In particular, we describe the pros and cons of each model approach. Results from simulations are presented to demonstrate the ability of the models to capture the known plasma and neutral dynamics near the three objects.     

Doppler Radar for Impact Drag Measurements

A Doppler radar tracking system has been used successfully to measure impact drag coefficients for several water-entry configurations. Hemisphere-cylinder and cone-cylinder models were launched vertically into a tank of water at velocities between 100 and 200 feet per second. These launchings were evaluation tests for a system to be used in a new facility at the Naval Ordnance Laboratory?the Hydroballistics Tank. Planned launchings in that facility will be at velocities up to 3000 feet per second. Knowledge of the drag coefficient profile (CD versus depth of penetration) is important in the design of high-velocity water-entry weapons.     

Statistically Optimum Optical Data Processing with Automatic Focus Estimation

The problem of optimally processing data with unknown focus is investigated. Optimum data processors are found by the method of maximum likelihood under a variety of assumptions that apply to most of the situations arising in practice. The unknown focus may be either an unknown parameter or an unknown random variable; the signal may be of known form or a random function; it is further assumed that the signal is received in additive, white, Gaussian noise. The problems of jointly estimating other unknown parameters and, in the case of a random signal, jointly estimating the signal, are also treated. The asymptotic variance and correlation of the estimators is discussed. Electrooptical realizations of the maximum likelihood computers are given. An iterative method of solution of the likelihood equation is also discussed. The discussion and results are directly applicable to the processing of synthetic aperture radar data.     

Toward an Understanding of the Systematic Uncertainties in Deriving the Primordial Helium Abundance from H II Region Observations

Recent determinations of the primordial He abundance have given significantly different results. We are attempting to identify some of the causes of these differences and propose observational solutions. Here we identify a systematic difference in how the data are interpreted (differences in corrections for the presence of neutral helium) and the importance of a systematic bias towards lower derived helium abundances (underestimating the presence of underlying stellar absorption).     

The performance of KSC Fixation Tubes with RNALater for orbital experiments: A case study in ISS operations for molecular biology

Molecular biology experiments on the International Space Station (ISS) continue to face challenges of sample harvesting and sample return to earth for post flight analysis; however, the use of Kennedy Space Center Fixation Tubes filled with RNALater has proven to be a robust solution to many of these challenges. While it is clear that one direction of future spaceflight experimentation may be towards enhanced on-orbit analytical capabilities, the rapid progress of earth-bound analytical capacity dictates that facile return of molecular biology samples from the ISS will continue to be a mainstay of space life sciences research and flight operations. In this paper we present a case study of the successful performance of KFTs and RNALater over a broad set of operational conditions of ascent configuration, on-orbit experiment use, on-orbit storage and sample return configurations that are unique to ISS current operations and constraints. We also provide observations on performance limits and discuss deployment opportunities and scenarios that are consistent with continued successful ISS molecular biology experimentation.